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E-Liquid Testing

October 17th, 2014


Component / Element
Protocol MDL
Tolerance
Nicotine Concentration
GC/FID Analysis 0.1 mg/ml
± 10%
Diethylene glycol (DEG)
GC/FID Analysis 2.23 µg/ml (ppm)
< 100 µg/ml (ppm)
Acetaldehyde
HPLC/UV Analysis 0.640 µg/ml (ppm)
< 100 µg/ml (ppm)
Diacetyl (2,3-Butanedione)
HPLC/UV Analysis 0.742 µg/ml (ppm)
Non-Detection Goal
< 22 µg/ml - Acceptable Levels
< 100 µg/ml (ppm) - Acceptable,but must be disclosed
> 100 µg/ml (ppm) is over suggested levels
Formaldehyde
HPLC/UV Analysis 0.846 µg/ml (ppm)
< 30 µg/ml (ppm)
Acetyl Propionyl (2,3-Pentanedione)
HPLC/UV Analysis 0.840 µg/ml (ppm)
Non-Detection Goal
< 22 µg/ml Acceptable Levels
< 100 µg/ml (ppm) - Acceptable,but must be disclosed
> 100 µg/ml (ppm) is over suggested levels
pH Level
Karl Fischer Analysis
Target Levels (Informational) 5.0 to 8.5
% Water
Karl Fischer Analysis
None Set (Informational)
Acetoin
HPLC/UV Analysis 0.841 µg/ml (ppm)
None Set (Informational)

Flavour
Wonderland
Cloudy With a Chance of Melons
Carnival
Sample ID
ECTA020-001
ECTA020-002
ECTA020-003
Enthalpy Code
0914-177-01
0914-177-02
0914-177-03
Nicotine Concentration
ND
10.7 mg/mL (±1.8%)
23.4 mg/mL (±3.2%)
Diethylene glycol (DEG)
ND
ND
ND
Acetaldehyde
9.20 µg/ml
2.2 J µg/ml
13.4 µg/ml
Diacetyl
ND
ND
3.39 µg/ml
Formaldehyde
1.53 µg/ml
1.51 µg/ml
1.40 µg/ml
Pentanedione
18.7 µg/ml
ND
9.71 µg/ml
pH Level
7.02
9.08
8.85
% Water
2.87%
2.57%
0.72%
Acetoin
72.3 µg/ml
4.00 µg/ml
49.7 µg/ml

ND: Non Detect or analytical result below the MDL.
J: Results flagged "J" are below the Lower Curve Limit, above the MDL, and are considered estimates.

Flavour
Jamakin me Blonde
Dragon's Blood
Double Dutch
Sample ID
ECTA020-004
ECTA020-005
ECTA020-006
Enthalpy Code
0914-177-04
0914-177-05
0914-177-06
Nicotine Concentration
5.94 mg/mL (±3.3%)
18.3 mg/mL (±0.8%)
12 mg/mL (±2.6%)
Diethylene glycol (DEG)
ND
ND
ND
Acetaldehyde
8.04 µg/ml
7.12 J µg/ml
7.14 µg/ml
Diacetyl
8.97 µg/ml
3.32 µg/ml
6.78 µg/ml
Formaldehyde
2.43 µg/ml
1.65 µg/ml
2.14 µg/ml
Pentanedione
20.0 µg/ml
ND
78.6 µg/ml
pH Level
7.95
8.35
8.32
% Water
0.95%
1.74%
1.26%
Acetoin
299 µg/ml
95.8 µg/ml
95.8 µg/ml

ND: Non Detect or analytical result below the MDL.
J: Results flagged "J" are below the Lower Curve Limit, above the MDL, and are considered estimates.

Flavour
St Cherry de Ha-Ha
Key West
Tequila Sunrise
Sample ID
ECTA020-007
ECTA020-008
ECTA020-009
Enthalpy Code
0914-177-07
0914-177-08
0914-177-09
Nicotine Concentration
18.5 mg/mL (±1.5%)
26.5 mg/mL (±3.6%)
12.9 mg/mL (±0.9%)
Diethylene glycol (DEG)
ND
ND
ND
Acetaldehyde
4.12 µg/ml
9.12 µg/ml
15.7 µg/ml
Diacetyl
ND
3.91 µg/ml
5.96 µg/ml
Formaldehyde
2.84 µg/ml
2.19 µg/ml
1.89 µg/ml
Pentanedione
3.28 µg/ml
6.86 µg/ml
23.8 µg/ml
pH Level
9.11
8.77
8.43
% Water
3.33%
1.78%
1.91%
Acetoin
ND
101
103

ND: Non Detect or analytical result below the MDL.
J: Results flagged "J" are below the Lower Curve Limit, above the MDL, and are considered estimates.

Flavour
Great Wall of Berries
Kookie Monster
Raffaelo
Sample ID
ECTA020-010
ECTA020-011
ECTA020-012
Enthalpy Code
0914-177-10
0914-177-11
0914-177-12
Nicotine Concentration
12.4 mg/mL (±0.7%)
6.26.5 mg/mL (±4.1%)
25.6 mg/mL (±0%)
Diethylene glycol (DEG)
ND
ND
ND
Acetaldehyde
5.21 µg/ml
11.4 µg/ml
12.3 µg/ml
Diacetyl
ND
7.22 µg/ml
11.0 µg/ml
Formaldehyde
2.14 µg/ml
1.63 µg/ml
1.24 µg/ml
Pentanedione
ND
112 µg/ml (Actions Taken)
55.3 µg/ml
pH Level
8.74
7.87
8.51
% Water
2.74%
1.70%
2.38%
Acetoin
105
89.8
109

ND: Non Detect or analytical result below the MDL.
J: Results flagged "J" are below the Lower Curve Limit, above the MDL, and are considered estimates.
Actions Taken
Levels of Pentanedione failed for Kookie Monster. We reformulated the e-juice as soon as the results came back and it was resent for testing. Judging by what the flavour extract company says - the new formnula will pass.
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